Radovi objavljeni u naučnim časopisima https://repo.niv.ns.ac.rs/xmlui/handle/123456789/4 2026-09-14T08:44:06Z 2026-09-14T08:44:06Z Rodrigues, Mariana Pozio, Edoardo Veneziano, Vincenzo Jansen, Famke Lazou, Thomai Dan, Sorin Daniel Rozycki, Miroslaw Petrović, Jelena Vieira-Pinto, Madalena https://repo.niv.ns.ac.rs/xmlui/handle/123456789/1163 2026-09-10T11:03:14Z 2026-01-01T00:00:00Z

Trichinella detection in wild boar meat intended for private consumption: Insights from a European study Rodrigues, Mariana; Pozio, Edoardo; Veneziano, Vincenzo; Jansen, Famke; Lazou, Thomai; Dan, Sorin Daniel; Rozycki, Miroslaw; Petrović, Jelena; Vieira-Pinto, Madalena Private consumption of wild boar meat is increasingly widespread across Europe. Notably, only a small proportion of hunted game is processed through approved establishments, while the majority is intended for private consumption. Testing wild boar meat for Trichinella spp. is mandatory when the meat is placed on the market. However, for private consumption this testing may not be carried out, raising public health concerns due to the risk of Trichinella spp. infection. To assess this risk, a survey was conducted in 26 European countries to collect data on the number of hunted wild boars (Sus scrofa) intended for private consumption and the prevalence of Trichinella spp. in wild boars, and to compare legislation and operational practices related to testing requirements, sample handling, diagnostic methods, training requirements, reporting procedures, mandatory measures for positive samples, penalties for non-compliance, and awareness-raising activities. Findings revealed significant heterogeneity in testing obligations, sample handling procedures, laboratory requirements, and enforcement mechanisms. While some countries mandate systematic testing and subsidize costs, others rely on voluntary testing or impose no requirements, leaving consumers at higher risk. Reported prevalence of Trichinella spp. in wild boar varied widely, ranging from 0% to over 6% across countries and years, though data availability was often inconsistent. Awareness campaigns and hunter training remain limited in many regions, contributing to underreporting, low compliance, and insufficient risk perception. Key challenges include lack of hunter engagement, insufficient testing facilities, and variable penalties for non-compliance. To mitigate risks, we recommend harmonizing surveillance strategies, expanding education and communication initiatives, ensuring accessible and affordable testing, and strengthening collaboration between veterinary authorities and hunting communities. Such measures are essential to reduce the zoonotic risk of trichinellosis linked to privately consumed wild boar meat in Europe.

2026-01-01T00:00:00Z Milanov, Dubravka Pajić, Marko Stefanović, Darko Vranešević, Jelena https://repo.niv.ns.ac.rs/xmlui/handle/123456789/1158 2026-09-08T08:02:38Z 2026-01-01T00:00:00Z

Salmonella Senftenberg: A Highly Adaptable Serovar Across Poultry Production and the Food Chain Milanov, Dubravka; Pajić, Marko; Stefanović, Darko; Vranešević, Jelena Salmonella enterica subspecies enterica serovar Senftenberg comprises a heterogeneous group of nontyphoidal Salmonella strains frequently detected in animal feed, feed ingredients, feed-manufacturing facilities, poultry production, and a wide range of foods. Genomic analyses indicate that S. Senftenberg is polyphyletic, comprising multiple genetically distinct evolutionary lineages and considerable phenotypic variation among isolates. Certain strains exhibit remarkable tolerance to heat, desiccation, and routine cleaning and disinfection procedures, facilitating their persistence in these environments and potentially contributing to transmission through the food chain. Human infections associated with S. Senftenberg range from self-limiting gastroenteritis to invasive disease, while some outbreak-associated isolates have demonstrated resistance to antimicrobials of particular importance in human medicine, adaptation to plant-derived antimicrobial compounds, and unusual virulence profiles. This review examines S. Senftenberg as an example of how adaptive traits can contribute to the persistence of Salmonella under environmental and selective pressures, with emphasis on its occurrence and persistence in poultry production and implications for food safety and public health.

2026-01-01T00:00:00Z Gajdov, Vladimir Pušić, Ivan Savić, Sara Lazić, Gospava Žekić, Marina Polaček, Vladimir Petrović, Tamaš https://repo.niv.ns.ac.rs/xmlui/handle/123456789/1153 2026-09-08T08:01:34Z 2026-01-01T00:00:00Z

Natural Infection of Domestic Dogs with Raccoon Dog and Fox Amdoparvovirus Resulting in a Severe Disease Outbreak Gajdov, Vladimir; Pušić, Ivan; Savić, Sara; Lazić, Gospava; Žekić, Marina; Polaček, Vladimir; Petrović, Tamaš Raccoon dog and fox amdoparvovirus (RFAV) has been reported in raccoon dogs and foxes, but natural infection in domestic dogs has not previously been documented. During March–April 2026, samples from four affected Dobermann dogs from a kennel near Novi Sad, Serbia, were submitted for laboratory investigation. After negative testing for canine adenovirus, canine coronavirus, herpesvirus, parvovirus, distemper virus, influenza A virus, and leptospirosis, metagenomic sequencing was performed on selected tissues, followed by bioinformatic analysis and targeted RFAV PCR screening of additional outbreak-associated samples. Affected dogs had prolonged illness characterized by conjunctivitis with ocular and nasal discharge, occasional blue eye appearance, progressive weight loss, poor coat quality, jaundice and biochemical evidence of hepatic injury, and neurologic signs including paraplegia in advanced cases. Sequencing generated 434,220 reads and identified multiple RFAV hits; pooled assembly produced a 4799 bp consensus genome with approximately 97% similarity to known RFAV strains and genome organization consistent with the genus Amdoparvovirus. RFAV DNA was subsequently detected by virus-specific PCR in an epidemiologically linked dog and across diverse specimen types including blood, urine, kidney, spleen, brain, lung, testicle, ileocecal lymph node, and throat swabs, whereas clinically healthy unrelated dogs were PCR-negative.

2026-01-01T00:00:00Z Galić, Ivan Spasojević, Jovan Đurđević, Biljana Lazić, Tatjana Kukurić, Tijana Popović, Nenad Stančić, Ivan Kovačević, Zorana https://repo.niv.ns.ac.rs/xmlui/handle/123456789/1150 2026-08-10T07:33:06Z 2026-01-01T00:00:00Z

Male Pseudohermaphroditism with Clitoromegaly in a Dog: Diagnostic Evaluation and Surgical Correction Galić, Ivan; Spasojević, Jovan; Đurđević, Biljana; Lazić, Tatjana; Kukurić, Tijana; Popović, Nenad; Stančić, Ivan; Kovačević, Zorana Background: Hermaphroditism, or intersexuality, is a pathological condition in which an individual possesses both male and female reproductive organs. Hermaphroditism is classified as true or pseudo-hermaphroditism. True hermaphrodit-ism is referred to an intersex condition in which an ovotestis is present. Pseudohermaphroditism is pathological condition in which an individual has gonads of one sex but the external genitalia of the opposite sex. This case report describes diagnostic procedures, surgical intervention and pathohistological findings in dog with male pseudohermaphroditism and clitoromegaly with penisoid appearance.Case: A 6-month-old mixed-breed dog was brought to the University Veterinary Clinic (Department of Veterinary Medi-cine, Faculty of Agriculture, University of Novi Sad) due to frequent urination and licking the vulva. Clinical examination of the reproductive tract revealed an enlarged clitoris. Complete blood count and biochemical analysis of serum did not show any abnormalities in collected blood samples, while the concentrations of estradiol and testosterone were as follows: estradiol 28.5 pg/mL, testosterone 1.8 ng/mL. Castration and clitorectomy were scheduled for the following day. After the midline laparotomy, gonads corresponding macroscopically to testes were observed at the anatomical position of the ovaries. The os clitoris was carefully dissected from the clitoral tissue and completely removed. Pathohistological exami-nation of the left and the right gonads revealed tissue composed of tubules lined solely by Sertoli cells, with no evidence of spermatogenesis. Morphologically, the gonadal tissue (testis) is consistent with the “Sertoli-cell-only” syndrome. No ovarian tissue was identified histologically.Discussion: Pseudohermaphroditism is generally a rare condition associated with reproductive dysfunction in dogs. Diag-nosis of intersex conditions requires a combination of clinical examination, ultrasonography, radiology, endocrinology, and pathohistology, as it has been illustrated in this case. Pathohistological analysis of both gonads confirmed the diagnosis of male pseudohermaphroditism. Microscopically, the presence of Sertoli cells without spermatogenesis and an epididymis without spermatocytes is described as “Sertoli-cell-only” syndrome, indicating that the testes in this case were not func-tionally developed. Oviductal tissue was also observed histologically. In the serum samples of dog in our case report, the testosterone level was 1.8ng/mL, which probably originates from the secretory role of Leydig cells. On the other hand, since the pathohistological analysis of both gonads did not find ovarian tissue (ovotestis), the question remains is - how the estradiol level was 28.5 pg/mL. Pathophysiologically, level of estradiol can be partially explained by pathology of Leydig cells or other physiological sources of the organism. This case confirms that early recognition of intersex conditions pre-vents complications and improves patient quality of life. Reproductive capability is not expected in such dogs, and surgical intervention ensures a favorable prognosis and preserves quality of life. Male pseudohermaphroditism with pronounced clitoromegaly is unfrequently described in the literature, while recent reports have described this condition in pure-breed dogs but also in one mix-breed, as it is described in this case. In this case report, the symptoms were completely resolved and the animal remained in good general condition after the surgery

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