| dc.contributor.author | Todorović, Dalibor | |
| dc.contributor.author | Stojanov, Igor | |
| dc.contributor.author | Pajić, Marko | |
| dc.contributor.author | Knežević, Slobodan | |
| dc.contributor.author | Tomić, Zdravko | |
| dc.contributor.author | Suvajdžić, Ljiljana | |
| dc.contributor.author | Velhner, Maja | |
| dc.date.accessioned | 2019-11-01T10:28:42Z | |
| dc.date.available | 2019-11-01T10:28:42Z | |
| dc.date.issued | 2019-04 | |
| dc.identifier.isbn | 978-86-83115-36-5 | |
| dc.identifier.uri | https://repo.niv.ns.ac.rs/xmlui/handle/123456789/83 | |
| dc.publisher | SVD, Sekcija za zoonoze, Beograd | en_US |
| dc.source | Zbornik kratkih sadržaja, XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8-10. april 2019 | sr |
| dc.subject.classification | methicillin | En |
| dc.subject.classification | resistance | En |
| dc.subject.classification | Staphylococcus аurеus | En |
| dc.subject.classification | detection | En |
| dc.subject.classification | PCR | En |
| dc.title | Detection of methicillin resistent Staphylococcus Aureus originating from animals | en_US |
| dc.title.alternative | Detekcija meticilin rezistentnog Staphylococcus aureus poreklom od životinja | en_US |
| dc.title.alternative | | en_US |
| dc.type | Other | en_US |
| dcterms.abstract | The first semisynthetic penicillin (methicillin) resistant to enzyme penicillinase has
been used in Europe since 1959 due to the occurrence of Staphylococcus aureus
resistant to penicillin. Already in 1961 in the United Kingdom strains of
Staphylococcus aureus resistant to methicillin (MRSA) have emerged. MRSA strains
are classified into the most important pathogens of humans and animals, and have
been proven to have the ability to move from animals to humans and vice versa.
Detection of MRSA strain is performed according to the recommendations of the
Institute for Clinical and Laboratory Standards (CLSI) and European Committee on
Antimicrobial Susceptibility Testing (EUCAST). It is primarily important that
sampling of nasal mucosa are taken correctly from animals as well as dust samples
from the farm, and delivered to the laboratory in an appropriate transport medium. In
order to inhibit the growth of other bacteria and stimulate the growth of
Staphylococcus aureus, swabs are inoculated into the brain-heart broth with the
addition of 6% salt. For isolation of MRSA a modified columbium agar with the
addition of 5% defibrillated sheep blood is used. The following routine diagnostic
tests, such as rabbit plasma coagulation test, novobiocin and polymyxin B are also
included in diagnostic protocol. Staphylococcus aureus resistant to cefotaxime is an
indicator that the strain is perhaps MRSA. Therefore a definite confirmation is done
by polymerease chain reaction which is used for detection of the mecA gene
responsible for the resistance to methicillin antibiotic. | En |
