Praćenje antitela protiv virusa bolesti kvrgave kože goveda posle vakcinacije ELISA metodom

Abstract

Humoral immune response of cattle stimulated by the lumpy skin disease virus (LSDV) is not completely understood. There is little literature data on the methods of detection of antibodies and development of seroconversion in cattle after vaccination against lumpy skin disease. The aim of this study was to present the results of detection of antibodies against LSDV in vaccinated cattle obtained by ELISA method. This study included a total of 60 cows, and blood sampling was carried out several times after vaccination with OBP Lumpy Skin Disease vaccine. Blood sera of the same 20 cows were collected and examined on vaccination day (day 0), 30 and 60 days after vaccination. Tests were also performed in blood sera samples of 20 cows, collected three months after vaccination, as well as in the sera of 20 cows collected four months after vaccination. In this way, a total of 100 blood sera samples of cows were tested. A commercial ELISA kit ID Screen Capripox Double Antigen Multispecies, manufactured by IDvet (France), was used for the detection of antibodies in the blood sera of cows. The positive antibody finding by this kit is defined if sample to positive ratio (S/P) is higher than 30%. The highest number of cows (13 or 65%) that had positive antibody finding was determined 30 days after vaccination with S/P values from 29.97% to 170.47%. Positive antibody finding was detected in 7 cows (35%) 60 days after vaccination, with S/P values from 120.47% to 141.51%. Antibodies against LSDV were found in 8 cows (40%), both 3 and 4 months after vaccination and S/P values ranged from 58.36% to 249.47% 3 months after vaccination, while 4 months after vaccination S/P values ranged from 42.39% to 270.19%. Results of this study showed that it was not possible to detect antibodies against LSDV in all vaccinated cattle, which corresponds to the literature data that in the protection of cattle from the infection with LSDV, besides humoral immunity, the role of cell-mediated immunity is also very important.