| dc.description.abstract | The health safety of honey includes the correctness in terms of the presence of different
contaminants, which also implies the antibiotic residue. The presence of antibiotics in honey is
prohibited, and methods of food analysis are prescribed in order to reliably determine antibiotics in
food. In this paper the application of ELISA tests for determination of selected antibiotics and
sulfonamides in honey is shown. The possibility of using four ELISA tests for the analysis of
tetracyclines, streptomycines, chloramphenicol and sulfonamides was examined. Each test was
evaluated after application on honey samples spiked with standard solution of a particular analyte.
Samples were prepared according to the instructions of the ELISA test manufacturer referring to
honey. Results of investigation of all ELISA tests, except for sulphonamides, have shown satisfactory
accuracy (73‒111%) and precision (14‒16%). Recovery for sulfametoxypyridazin was low (40%), and
for low tetracycline concentration was somewhat higher than acceptable (139%). The detection limits
were in accordance with the limits given by the ELISA kit manufacturer and are also satisfactory in
relation to the requirements of the legislation (0.075‒3 μg/kg). The test kits can be used to screen the
presence of tetracycline, chloramphenicol and streptomycin in the honey, taking into account the
obtained validation parameters. | en_US |
