Validation of TaqMan-Based Assays for Specific Detection and Differentiation of Wild-Type and Neethling Vaccine Strains of LSDV
Date
2021Author
Vidanović, Dejan
Tešović, Bojana
Šekler, Milanko
Debeljak, Zoran
Vasković, Nikola
Matović, Kazimir
Koltsov, Andrey
Krstevski, Kiril
Petrović, Tamaš
De Leeuw, Ilse
Haegeman, Andy
Metadata
Show full item recordAbstract
Lumpy skin disease (LSD) is an important animal disease with significant health and
economic impacts. It is considered a notifiable disease by the OIE. Attenuated strains of LSDV
have been successfully used as vaccines (LAV) but can also produce mild or systemic reactions.
Vaccination campaigns using LAVs are therefore only viable if accompanying DIVA assays are
available. Two DIVA qPCR assays able to distinguish Neethling-based LAVs and wild-type LSDV
were developed. Upon validation, both assays were shown to have high sensitivity and specificity
with a diagnostic performance comparable to other published DIVA assays. This confirmed their
potential as reliable tools to confirm infection in animals during vaccination campaigns based on
Neethling vaccine strains.