Effect of an Antioxidant Supplement Combinationon Boar Sperm

Abstract

The studywasconductedonacommercialpigfarmlocatedinSerbia.ThirtyDurocor Landrace breedboarswererandomlyselectedforthisstudy.Theexperimentalgroupwasfeda compound feedwithaddedorganicseleniumandOxynat3D.Theantioxidantstatusparameters of boarseminalplasmawereevaluatedusingabiochemicalanalyzerandcommercialRandoxkits. The spermchromatinstructureassay(SCSA)usingflowcytometry(FC)providedinformationabout spermatozoa’s DNAstatus.Additionally,thetotalnumberofmotilespermatozoaandspermatozoa kinematic parametersweremeasuredusingthecomputer-assistedspermanalysis(CASA)system. The aimofthisstudywastoimprovetheparametersofsemenbycombiningtwopreparationsthat have apotentialantioxidanteffect,butalsotoestablishthelevelofvariousantioxidantenzymes in nativesperm.Therewasnostatisticallysignificantdifferenceintotalantioxidantcapacityand glutathione peroxidaseactivityintheseminalplasmaobtainedfromtheexperimentalandcon- trolgroupsofboars.Regardingthesuperoxidedismutaseactivity,theresearchresultsshoweda differenceinthecontrolgroupcomparedtotheexperimentalone.Moreover,spermatozoaDNA fragmentation andthetotalnumberofmotilespermatozoashowedstatisticallysignificantlower and highervalues,respectively,inexperimentalcomparedtothecontrolgroups.Thecombination of thesetwopreparationsshowssignificantlyenhancedvitalparametersofsemen.Tothebestof our knowledge,thisstudyisthefirstinwhichtheejaculateparameterswereexaminedafterthe application ofacombinationofthesetwoantioxidantsupplements.